Monitoring Dynamic Changes of Cellular Membrane GSH During Stroke via an ESIPT-Based Near-Infrared Fluorescent Probe

Monitoring Dynamic Changes of Cellular Membrane GSH During Stroke via an ESIPT-Based Near-Infrared Fluorescent Probe

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Stroke, primarily ischemic (85%), results from inadequate blood supply and is worsened by ferroptosis, characterized by free radical generation and lipid peroxidation.Monitoring ferroptosis is essential for understanding its mechanisms and developing treatments.Glutathione (GSH) is a key ferroptosis biomarker, but current probes are limited old taylor whiskey 1933 price by short excitation/emission wavelengths, small Stokes shifts, and inability to monitor dynamic GSH changes at the cellular membrane, where ferroptosis plays a crucial role.

To address these issues, we developed the PM-Red-GSH, a novel near-infrared (NIR) probe based on the Excited-state intramolecular proton transfer (ESIPT) mechanism.It shows strong NIR emission (715 nm), large Stokes shift (290 nm), and enhanced membrane binding (PCC = 0.95) due to its alkyl group.

PM-Red-GSH enables dynamic GSH monitoring in an MCAO mouse model.These findings offer new insights into canon imageclass mf227dw ferroptosis and stroke treatment.